AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)
DOI:
https://doi.org/10.37090/jfl.v8i1.81Abstract
Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-PicrilhydrazilReferences
Liwun, N.M. 2009. Inventarisasi dan Identifikasi Tanaman Obat Yang Digunakan Oleh Suku Dayak Lundayeh Di Kecamatan Mentarang Kabupaten Malinau, Kalimantan
Timur. Karya Tulis Ilmiah. Akademi Farmasi Samarinda: Samarinda.
Jubaidah, .S., Sapri., Supriningrum, R. 2013. Karakterisasi dan Skrining fitokimia Akar Tabar Kedayan (Aristolochia foveolata Merr.) Prosiding Seminar Nasional Kimia, Universitas Mulawarman, Samarinda.
Astuti Ambar Dwi Widhi. 2011. Efektivitas Pemberian Ekatrak Jahe Merah (Zingiber officinale roscoe varr Rubrum) Dalam Mengurangi Nyeri Otot Pada Atlet Sepak Takraw. Artikel Penelitian. Universitas Diponegoro. Semarang.
Jubaidah, S dan Nurhasnawati, H. 2018. Analisis Flavonoid Total Akar Tabar Kedayan (Aristolochia foveolata Merr.) Jurnal Al Kimia, Jurusan Kimia Universitas Islam Negeri Alaudin, Makasar.
Departemen Kesehatan RI. 2009. Farmakope Herbal Indonesia edisi pertama. Jakarta : Depkes RI
Departemen Kesehatan RI.1979. Materia Medika Indonesia jilid III. Jakarta : Dirjen Pengawas Obat dan Makanan.
Latifah. 2015. Identifikasi Golongan senyawa Flavonoid dan Uji Aktivitas Antioksidan Pada ekstrak Rimpang Kencur (Kaemferia galanga L.) Dengan Metode DPPH. Skripsi. Jurusan Kimia Fakultas Sains dan teknologi Universitas Islam Negeri Maulana Malik Ibrahim Malang.
Molyneux, P. 2004. “The Use Of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) For Estimating Antioxidant Activityâ€. Journals Songklanakarin Science Technology . 26 (2) : 211-219.
Gandjar, I. G, dan Abdul, R. 2007. Kimia Farmasi Analisis. Pustaka Pelajar: Yogyakarta
Rachmawati, IS dan Ciptati. 2011. Isolasi Senyawa Antioksidan dari Daun Sirih Merah (Piper crocatum). Bandung : Prosiding Simposium Nasional Inovasi Pembelajaran dan Sains.
