FRAKSI ETANOL EKSTRAK KULIT PISANG KEPOK KUNING ( Musa balbisiana) TERHADAP BAKTERI Staphylococcus epidermidis, Staphylococcus aureus, dan Propionibacterium acnes
DOI:
https://doi.org/10.37090/jfl.v10i1.492Abstract
Yellow kepok banana peel (Musa balbisiana) contains antibacterial compounds against S. epidermidis, S. aureus and P.acne. This study aims to proved the antibacterial activity of the yellow kepok banana peel extract fractionated using ethanol as a solvent against S.epidermidis, S.aureus and P.acne. The yellow kepok banana peel extraction process was carried out by maceration method with 70% ethanol followed by fractionation to obtain ethanol fraction. Antibacterial activity testing using nutrient agar (NA) media with wells method with a concentration of 25,000 ppm, 50,000 ppm, 100,000 ppm, aquadest as a negative control and clindamycin as a positive control. The phytochemical test of the ethanol fraction showed the content of alkaloids, flavonoids, saponins, tannins and quinones. The results of the inhibition zone diameter of the ethanol fraction at a concentration of 100,000 ppm against S. epidermidis (11.87 mm), S. aureus (12.04 mm) and P. acne (11.35 mm). The test is to determined the minimum inhibitory concentration (MIC) the minimum inhibitory concentration value of the ethanol fraction of S. epidermidis, S. aureus is 20,000 ppm and for P. acne is 17,500 ppm. The conclusion of this studied was that the ethanol fraction of kepok yellow banana peel extract has inhibitory and bacteriostatic properties against S. epidermidis, S. aureus and P.acne bacteria.
Keywords: alkaloids,Ant ibacter ial , flavonoids , Fractionated, Musa balbisiana
References
DAFTAR PUSTAKA
Saraswati FN. Uji Aktivitas Antibakteri Ekstrak Etanol 96% Limbah Kulit Pisang Kepok Kuning ( Musa balbisiana ) Terhadap Bakteri Penyebab Jerawat ( S. epidermidis, S. aureus, dan P.acne). 2015.
Maharani A. Penyakit Kulit: perawatan, Pencegahan, Pengobatan. Yogyakarta: Pustaka Baru press; 2015
Adhi Juanda, Mochtar Hamzah SA. Ilmu penyakit kulit dan kelamin. 6th ed. Jakarta: Badan Penerbit FKUI; 2011.
Ningsih AP, Nurmiati, Agustien A. Uji Aktivitas Antibakteri Ekstrak Kental Tanaman Pisang Kepok Kuning (Musa paradisiaca Linn.) terhadap S. aureus dan Escherichia coli. Biol Univ Andalas. 2013;2(3):207–13.
Harborne, J.B. 2006. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Edisi ke- 2. Penerbit ITB, Bandung.
J. A. Priyanto, S. Pujiyanto, and Rukmi, “Flavonoids Production Capability Test of Tea Mistletoe ( Scurrula atropurpurea BL . Dans ) Endophytic Bacteria Isolates Jurnal Sains dan Matematika,†JSM, vol. 22, no. 4, pp. 89–96, 2014.
Sugita, T.; Miyamoto, M.; Tsuboi R.; Takatori, K.; Ikeda, R. & Nishikawa A. In Vitro Activities of Azole Antifungal Agents againts P.acnes Isolated from Patients with Acne Vulgaris. Pharm Bull. 2010;33(1):125–127
American Society for Microbiology. Manual of antimicrobial susceptibility testing. Manual of antimicrobial susceptibility testing. 2005. 53-62 p.
Nilsson, Lars, Flock, Pei, Lindberg G. Fibrinogen-Binding Protein of S. epidermidis, Infection and Immunity. 1998;66(6):2666–2673.
Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. 2005;26:343–5
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