BIOAKTIVITAS ANTIOKSIDAN SENYAWA EKSOPOLISAKARIDA DARI MIKROALGA Dunaliella sp.
DOI:
https://doi.org/10.37090/jfl.v6i2.18Abstract
A study of antioxidant compounds in microalgae, Dunaliella sp. In Conway / Walne media. Microscope observation with magnification 400 times obtained diameter Dunaliella sp. Sebebsar 6.42 μm. Preliminary results of antioxidant activity using DPPH reagent showed Dunaliella sp. Excreting antioxidant compounds. The extract obtained by extracting antioxidant compounds exopolysaccharide compounds from the filtrate with the addition of 70% ethanol and then centrifuged and freeze-dried by the method of FTIR spectrum data dry. Unidentified exopolysaccharide compounds containing uptake helping O - H in the area of 3544.82 cm-1, C - H in area of 2828.59 cm-1, helping the C • O at 1624.24 cm-1 region, CH2 bending at 1419.44 cm-1 region, and helping C - O at 1151.88 cm-1 region. Furthermore, quantitative test results of antioxidant compounds that have a value of 4.279%. So it is concluded that the exopolisakarida compound from Dunaliella sp. Is a potential source of antioxidant compounds. Keywords : Antioxidant, exopolisaccharide, Dunaliella sp.References
Sukara, E. 2006. Biotrends. Puslit Bioteknologi LIPI. Majalah Populer Bioteknologi. Vol. 1 No.2.
Richmond, A. and Qiang Hu. 2013. Handbook of Microalgal Culture Applied Phycology and Biotechnology. Wiley-Blackwell.
Anderson, R.A. 2005. Algae Culturing Technique. Elsevier Academic Press. London.
Barsanti, Laura and Paolo Gualtieri. 2006. Algae Anatomy, Biochemistry and Biotechnology. Taylor & Francis Group, the academic division of T&F Informa plc.
Pisal S Dipak dan S S lele. 2005. Carotenoid from Microalga, Dunaliella salina. Biotechnology. 476-483.
Wu Q., Cheng Zheng, Zheng-Xiang Ning and Bao Yang. 2007. Modification of Low Molecular Weight Polysaccharides from Tremella Fuciformis and Their Antioxidant Activity in Vitro. J. Mol. Sci. 2007, 8, 670-679.
GarcÃa-González, M, Moreno, J, Manzano, J.C, Florêncio, F.J, Guerrero, M.G. 2005. Production of Dunaliella salina biomass rich in 9-cis-β-carotene and lutein in a closed tubular photobioreactor. J. Biotechnol. 115, 81–90.
Harutoshi, T. 2013. Exopolysaccharides of Lactic Acid Bacteria for Food and Colon Health Applications. licensee InTech.
Nwodo, U. U., Ezekiel Green and Anthony I. Okoh. 2012. Bacterial Exopolysaccharides: Functionality and Prospects. Int. J. Mol. Sci. 13, 14002-14015.
Sajna, K. V., Rajeev K. Sukumaran, Lalitha Devi Gottumukkala, Himani Jayamurthy, Kiran S. Dhar, Ashok Pandey. 2013. Studies on structural and physical characteristics of a novel exopolysaccharide from Pseudozyma sp. NII 08165. International Journal of Biological Macromolecules. 84– 89
Molyneux, R.J. 2009. Bioactive Natural Product, Detection, Isolation and Structural Determination 2nd Edition. CRC Press. Taylor and Francis. United States.
