AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL 70% DAUN ASHITABA (Angelica keiskei) SECARA IN VIVO DENGAN PENGINDUKSI KARAGENAN
DOI:
https://doi.org/10.37090/jfl.v7i2.56Abstract
Ashitaba leaves (Angelica keiskei) or also known as tommorow's leaf is plant that known to have various health benefit, one of them is as an anti-inflammatory activity. The anti-inflammatory activity of ashitaba leaves has been known through in vitro assays. This study aims to determine the anti-inflammatory activity of 70% ethanol extract of ashitaba leaves through in vivo assay. Anti-inflammatory activity was performed on white male rat of Sprague dawley strain with induction method of edema on rat's foot using 1% carrageenan 0.2 ml. Rats were divided into 5 groups. The negative control group was given a 0.5% Na-CMC suspension, a positive control group was given sodium diclofenac suspension of 5.14 mg / kgBW, and the test group was given 70% ethanol extract of ashitaba leaves at a dose of 1000; 2000; and 4000 mg / kgBW suspended in 0.5% Na-CMC. The results showed that in that dose range the 70% ethanol extract of ashitaba leaves had anti-inflammatory activity that did not depend on the dose. Percentage of edema of 70% ethanol extract of ashitaba leaves dose 1000; 2000; 4000 mg / kgBB was significantly different with negative control (p ≤ 0,05) and had percentage of edema inhibition respectively 83,95%, 79,01%, and 80,25%. The results of this study showed that 70% ethanol extract of ashitaba leaves have anti-inflammatory activity. Keywords: Ashitaba, Angelica keiskei, tommorow's leaf, anti-inflammatory, carrageenan.Downloads
References
Tilo Grosser, Emer Smith GAF. Goodman & Gilman’s. 2011. The Pharmacological Basis of Therapeutics. p. 959–1000.
Inamori Y, Baba K, Tsujibo H, Taniguchi M, Nakata K, Kozawa M. 1991. Antibacterial Activity of Two Chalcones, Xanthoangelol and 4-Hydroxyderricin, Isolated from the Root of Angelica keiskei Koidzumi. Chem Pharm Bull. 39(6):1604–5.
Matsuura M, Kimura Y, Nakata K, Baba K, Okuda H. 2001. Artery Relaxation Chalcones Isolated from the Roots of Angelica keiskei. Planta Med. 2000;67:230–5.
Ugiyama KAS, Anabe MAT, Obayashi EIJIK. 2007. Antidiabetic Activities of Chalcones Isolated from a Japanese Herb , Angelica keiskei. J.Agric FoodChem. 55:6013–7.
Kimura Y. 2008. Antitumor and Antimetastatic Actions of Various Natural Products. Stud Nat Prod Chem. 34(C):35–76.
Kwon D, Yoon S, Carter O, Bailey GS. 2006. Antioxidant and antigenotoxic activities of Angelica keiskei , Oenanthe javanica and Brassica oleracea in the Salmonella mutagenicity assay and in HCT116 human colon cancer cells. Bio Farctors .26.26:231–44.
Lee HJ, Kim HJ, Choi S, Kim S, Sethi G, Ahn KS. 2010. 1 1 1 1. ;13(3):691–9.
Caesar LK, Cech NB. 2006. A Review of the Medicinal Uses and Pharmacology of Ashitaba. Planta med.
Hsieh H, Tsao L, Wang J. 2006. Synthesis and Anti-in ¯ ammatory Effect of Chalcones. J Pharm Pharmacol. 52:163–71.
Winter CA, Risley EA, Nuss GW. 1962. Carrageenin-Induced Edema in Hind Paw of Rat as an Assay for Antiinflammatory Drugs. Exp Biol Med. 1–5.
Morris CJ. 2003. Carrageenan-induced paw edema in the rat and mouse. Methods Mol Biol. 225:115–21.
