AKTIVITAS ANTIBAKTERI DARI FRAKSI DAUN KATUK HUTAN (Breynia cernua) ASAL PAPUA
ANTIBACTERIAL ACTIVITY OF FRACTION KATUK HUTAN LEAF (Breynia cernua) FROM PAPUA
DOI:
https://doi.org/10.37090/jfl.v14i1.2489Abstrak
Daun katuk hutan (Breynia cernua) merupakan tanaman endemik Papua secara tradisional digunakan untuk mengobati kanker, cacar dan luka. Tanaman ini diketahui mengandung berbagai senyawa bioaktif seperti alkaloid, flavonoid, terpenoid dan tanin. Fraksinasi dilakukan menggunakan pelarut n-heksana, etil asetat dan etanol-air untuk memperoleh komponen bioaktif yang lebih spesifik. Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri fraksi ekstrak daun Breynia cernua terhadap Staphylococcus aureus dan Escherichia coli. Pengujian antibakteri dilakukan dengan metode difusi cakram pada konsentrasi 250, 500 dan 750 ppm. Semua fraksi menunjukkan zona hambat dalam kategori sedang hingga kuat terhadap kedua bakteri uji. Aktivitas antibakteri tertinggi ditunjukkan oleh fraksi n-heksana pada konsentrasi 750 ppm dengan zona hambat sebesar 10,2 ± 0,8 mm terhadap S. aureus dan 10,3 ± 0,8 mm terhadap E. coli. Hasil analisis ANOVA menunjukkan adanya perbedaan yang signifikan (p < 0,05) antara fraksi n-heksana dan fraksi lainnya. Temuan ini menunjukkan bahwa fraksi n-heksana dari daun Breynia cernua memiliki efek antibakteri paling kuat.
Unduhan
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