UJI TOKSISITAS EKSTRAK ETANOL DAUN JAMBU BIJI AUSTRALIA (Psidium guajava L) DENGAN METODE BSLT (Brine Shrimp Lethality Test)
DOI:
https://doi.org/10.37090/jfl.v9i1.326Abstrak
ABSTRACT
Guava is one of the plants that can traditionally be used for the treatment of diseases. Many kinds of guava, one of which is the Australian guava has the characteristics of roots, stems, leaves, dark red fruit. This study aims to determine the toxicity of the ethanol extract of Australian guava leaves (Psidium guajava L) using the BSLT (Brine Shrimp Lethality Test) method and determine the chemical content of Australian guava leaves (Psidium guajava L). The extract was made by the ultrasonic method using 96% ethanol solvent. Toxicity tests were carried out using 48-hour-old Artemia salina Leach shrimp larvae. The toxic effect of the extract was identified by the percentage of shrimp larvae mortality using probit analysis (LC50). From the research results, phytochemical content includes tannins, flavonoids, alkaloids, terpenoids and saponins, and flavonoid compounds have the highest content compared to the others. Research shows that the ethanol extract of Australian guava leaves is of a moderate category (LC50 441,977 ppm).
Keywords :Australia guava leaves, BSLT, Artemia salina L, Ultrasonic
Referensi
Daftar Pustaka
Ahmad AR, Juwita J, Ratulangi SAD, Malik A. 2016. Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patilaka (Etlingera elatior (jack) Rm Sm) Menggunakan SpektrofotometriUV-Vis. Pharmaceutical Sciences And Research (Psr), 2(1), Pp 1-10.
Cahyadi R. 2009. Uji Toksisitas Aku Ekstrak Etanol Buah Pare (Momordika charantia L) Terhadap Larva Artemia salina L Dengan Metode BSLT [Skripsi]. Semarang: UNDIP.
Chang C, Yang M, Wen Hand Chern J. 2002. Estimation of the Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. J. Food Drug Anal.
Dwitiyanti. 2015. Daun Jambu Biji (Psidium guajava L) sebagai Antikanker Payudara. [Skripsi]. Jakarta Timur: UM Prof. DR. Hamka.
Handayani H, Sriherfyna FH. 2016. Ekstraksi Antioksidan Daun Sirsak Metode Ultrasonik Bath (Kajian Rasio Bahan : Pelarut dan Lama Ekstraksi). Jurnal Pangan dan Agroindustri 4(1) : 262-272.
Harborne JB. 1897. Metode Fitokimia. “Penuntun cara modern menganalisa tumbuhan†Terbitan kedua. Bandung: ITB.
Herawati N, Jalaluddin N, Daha L, Firdaus Z. 2009. Sonneratia alba sebagai sumber senyawa antibakteri potensial. Jurnal Indonesia Chemica Acta. 2(2): 10-16.
McLaughlin JL, Rogers LL, Anderson JE. 1998. The Use Of Biological Assays To Evaluate Botanicals. Drug Inform JL. Vol 32. Meyer BN. 1982.
Brine Shimpe, A: “Convenient General Bioassay for active Plant Constituentâ€. Plant Medica.
Parimin SP. 2005. Jambu Biji: Budidaya dan Ragam Pemanfaatannya. Jakarta: Penebar Swadaya.
Rusdi. 1990. Tetumbuhan Sebagai Sumber Bahan Obat. Padang : Pusat Penelitian Universitas Andalas
