PENAMBAHAN TREHALOSE DALAM PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SEMEN SAPI PESISIR
DOI:
https://doi.org/10.37090/jwputb.v7i2.1014Abstract
The purpose of this study was to find out how the semen quality of pesisir cattle was after the addition of trehalose to egg yolk tris diluent. The research material in the form of fresh semen was obtained from pesisir cattle aged approximately 4 years and the quality of the semen had been tested by observing the quality of fresh semen before dilution.. This study used a randomized block design with 4 treatments and 4 replications. The treatment given was the addition of trehalose in egg yolk tris diluent. The treatment given consisted of control, the addition of trehalose 0.5%, 1%, and 1.5%. The variables in this study included motility, viability, intact plasma membrane (MPU), and intact acrosome cap (TAU) of spermatozoa of pesisir cattle. The results of this study indicated that the addition of trehalose had a significant effect (p<0.05) on the viability, MPU, TAU, and did not show a significant effect (p>0.05) on the motility of spermatozoa of pesisir cattle. The results of this study indicated that the dose of trehalose addition of 1.5% in egg yolk tris diluent showed the best results on the quality of spermatozoa of pesisir cattle, where motility was found to be 53%, viability 61%, MPU 63%, and TAU 63%.
Keywords : Trehalose, Pesisir Cattle, Semen Quality of Pesisir Cattle.
References
Aboagla, E. M. E dan T. Terada, 2004. Effects of suplementation of trehalosa ext ender containing egg yolk with sodium dodecyl sulfate on the freezability of goat spermatozoa. Theriogenology. 45: 513-520.
Afriani, T., Y. M. Pela, E. Roza, A. Rastosari, and A. Farhana. 2022. Addition of Trehalose of Duck Egg Yolk-Tris as an Extender Medium on Buffalo Frozen Semen. In International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021). Atlantis Press. pp. 23-27.
Aisen, E. G., V. H. Medina and A. Venturino. 2002. Cryopreservation and post-thawed fertility of ram frozen semen in different trehalose concentratons. Theriogenology. 58:1801-1808.
Badr, M. R., G. A. Mary, and M. H. Hassan. 2010. Effect of Trehalose on Cryopreservation, Oxidative Stress and DNA Integrity of Buffalo Spermatozoa. J Reprod Infert. 1 (2) : 50-57.
Badan Pusat Statistik Kabupaten Pesisir Selatan. 2022. Kabupaten Pesisir Selatan Dalam Angka.
Bansal, A.K dan G.S. Bilaspuri. 2009. Antioxidant effect of vitamin e on motility, viability and lipid peroxiation of Cattle spermatozoa under oxidative stress. Animal science papers and reports, 27(1):5-14.
Bansal, A. K., and G. S. Bilaspuri. 2011. Impacts of oxidative stress and antioxidants on semen functions. Veterinary medicine international. 2011.
Celeghini ECC, Nascimento J, Raphael CFA, Andrade AFC, Arruda PR. 2010. Simultaneous assessment of plasmatic, acrosomal, and mitochondrial membranes in ram sperm by fluorescent probes. Arquivo Brasileiro de Medicina Veterináriae Zootecnia 62(3): 536-543.
Chhillar, S., V. K. Singh, R. Kumar, S. K. Atreja. 2012. Effects of Taurine or Trehalose Supplementation on Functional Competence of Cryopreserved Karan Fries Semen. Anim Reprod Sci. 135 : 1-7.
El-Sheshtawy, R. I., G. A. Sisy, and W. S. El-Nattat. 2015. Effects of Different Concentrations of Sucrose or Trehalose on the Post-Thawing Quality of Cattle Bull Semen. Asian Pacific Journal of Reproduction. 4 (1) : 26-31.
Gibb, Z., and R. J. Aitken. 2016. The Impact of Sperm Metabolism during In Vitro Storage: The Stallion as a Model. BioMed Res. Int. 2016 : e9380609.
Hikmawan, S.W., G. Cipatadi,dan S. Wahjuningsih. 2016. Kualitas Spermatozoa Swim Up Kambing Peranakan Etawah Hasil Pembekuan Menggunakan Metode Vitrifikasi dengan Persentase Gliserol yang Berbeda. Jurnal Ternak Tropika. 17(1).
Herdis, M. Surachman , M. Rizal, A. Boediono dan Yulnawati. 2006. Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries). Biosfera 23 (1) : 24-30.
Hu, J. H., L. S. Zan, X. L. Zhao, Q. W. Li, Z. L. Jiang, Y. K. Li, and X. Li. 2010. Effects of Trehalose Supplementation on Semen Quality and Oxidative Stress Variables in Frozen-Thawed Bovine Semen. J Anim Sci. 88 (5) : 1657-1662.
Iqbal, S., Naz, S., Ahmed, H., & Andrabi, S. M. H. (2018). Cryoprotectant effect of trehalose in extender on post‐thaw quality and in vivo fertility of water buffalo (Bubalus bubalis) bull spermatozoa. Andrologia, 50(1), e12794.
Iqbal, S., S. M. H. Andrabi, A. Riaz, A. Z. Durrani, and N. Ahmad. 2016. Trehalose Improves Semen Antioxidant Enzymes Activity, Post Thaw Quality, and Fertility in Nili Ravi Buffaloes (Bubalus bubalis). Theriogenology. 85 : 954–959.
Jha, P. K., M. G. S. Alam, M. A. A. Mansur, M. R. I. Talukder, N. Naher, A. K. M. A. Rahman, D. C. Hall, and F. Y. Bari. 2020. Effects of Number of Frozen-Thawed Ram Sperm and Number of Inseminations on Fertility in Synchronized Ewes Under Field Condition. J Anim Reprod Biotechnol. 35 (2) : 190-197.
Maia, M. S., S. D. Bicudo, and L. Rodello. 2014. Effect of Hydrogen Peroxide on Thawed Ovine Sperm Motility. Anim. Reprod. 11 : 119–123.
Oztürk, A. E., M. N. Bucak, M. Bodu, N. B. pınar, I. Çelik, Z. Shu, N. Keskin, and D. Gao. 2019. Cryobiology and Cryopreservation of Sperm. Cryopreservation. Current Advances and Evaluations. 2020.
Pelufo, V., M. L. Armengol, V. Malcotti, A. Venturino, and E.G. Aisen. 2015. Effects of Glycerol and Sugar Mixing Temperature on the Morphologic and Functional Integrity of Cryopreserved Ram Sperm. Theriogenology. 83 : 144–151.
Reddy, N. S. S., G. J. Mohanarao, and S. K. Atreja. 2010. Effects of Adding Taurine and Trehalose to A Tris-Based Egg Yolk Extender on Buffalo (Bubalus bubalis) Sperm Quality Following Cryopreservation. Anim Reprod Sci. 119 : 183-190.
Rickard, J. P., T. Pini, C. Soleilhavoup, J. Cognie, R. Bathgate, G. W. Lynch, G. Evans, W. M. Maxwell, X. Druart, and S. P. de Graaf. 2014.Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa. Reproduction. 148 (5) : 469-478.
Sarbaini. 2004. Kajian Keragaman Karakteristik Eksternal dan DNA Mikrosatelit Sapi Pesisir Sumatera Barat Disertasi Sekolah Pascasarjana IPB. Bogor
Singh, V. K., S. K. Atreja, R. Kumar, S. Chhillar, and A. K. Singh. 2012. Assessment of intracellular Ca 2+, cAMP and 1, 2-diacylglycerol in cryopreserved buffalo (bubalus bubalis) spermatozoa on supplementation of taurine and trehalose in the extender. Reprod Domst Anim. 47 : 584–590.
Tuncer, P. B., U. Taşdemir, S. Büyükleblebici, T. Özgürtaş, E. Coşkun, H. Erol, ... and I. S. Gürcan. 2013. Effects of Different Doses of Trehalose Supplementation in Egg Yolk Extender in Frozen–Thawed Angora Buck Semen. Small Ruminant Research. 113 (3) : 383-389.
Valente, S., R. Pereira, M. Baptista, C. Marques, M. Vasques, M. S. Pereira, A. Horta, and J. Barbas, 2010. In Vitro and In Vivo Fertility of Ram Semen Cryopreserved in Different Extenders. Anim. Reprod. Sci. 117 : 74–77.
Vrielink, A. S. O., A. Aloi, L. L. Olijve, and I. K. Voets. 2016. Interaction of Ice Binding Proteins With Ice, Water and Ions. Biointerphases 1 (1) : 018906.
Yurnalis. 2013. Polimorfisme Gen Hormon Pertumbuhan Pada Sapi Pesisir Sumatera Barat. Disertasi Program Pascasarjana Universitas Andalas.
Zhu, Z., X. Fan, Y. Pan, Y. Lu, and W. Zeng. 2017. Trehalose Improves Rabbit Sperm Quality During Cryopreservation. Cryobiology, 75, 45-51.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Auli Two Putra Syahminan, Jaswandi Jaswandi, Tinda Afriani
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
