Identifikasi Keragaman SNP Gen MSTN Pada Sapi Limousin
AbstractAbstract Limousin cattle (Bos Taurus) are beef cattle that have a large, long, body shape, with have advantages of fast body growth, high fertility and easy reproducibility. Myostatin gene is a family of TGF-β which plays a role in muscle growth and meat quality. This study aims to identify diversity of the Single Nucleotide Polymorphism (SNPs) Myostatine gene in the coding region in limousine cattle. Blood samples were taken from 15 limousine cattle from BPTU-HPT Padang Mangatas, West Sumatra Province. SNPs identification is finish by amplifying the coding region using the sequencing method. This study revealed 12 SNPs spread over 2 SNPs in exon 1 (c.400 G>A, c.415 C>A), 8 SNPs in exon 2 (c.2411 C>T, c.2477 G>A, c. .2489 G>A, c.2503 G>A, c.2521 G>A, c.2577 C>T, c.2609 G>A, c.2636 G>A), and in exon 3 find 2 SNPs (c .5107 C>A, c.5113 T>C) are polymorphic with allele frequencies in general being in Hardy-Weinberg equilibrium except for SNP c.2411 C>T which is not in balance. SNPs identified in the MSTN gene of limousine cattle are expected can fungtion as candidate genetic markers, especially for the perfection of beef cattle in Indonesia. Key words: Limousin Cattle, MSTN, SNP
Aliyya W L N, Noor RR, Jakaria. 2020. Exploring SNPs (Single Nucleotide Polymorphisms) of Myostatin gene in coding region in Bali Cattle. IOP International Conference of Animal Science and Technology. Vol. 492.
Allendrof FW, Luikart GH, Aitken SN. 2013. Conservation and the Genetics of Populations. Edisi ke-2. West Sussex (UK): Wiley-Blackwell Publishing.
Luo G, Li X, Han Z, Zhang Z, Yang Q, Guo H, Fang J. 2016. Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae). Nanjing (CN): Jiangsu Academy of Agricultural Sciences.
Meuwissen TH, Hayes BJ, Goddard ME. 2001. Prediction of total genetic value using genome-wide dense marker maps. Genetic journal. 157(4): 1819-1829.
Nei M, Kumar S. 2000. Molecular Evolution and Phylogenetics. New York (USA): Oxford Univ Pr.
Noor RR. 2010. Genetika Ternak. Edisi ke-6. Jakarta (ID): Penebar Swadaya.
Oldham JM, Martyn JAK, Sharma M, Jaenplong F, Kambadur R, Bass JJ. 2001. Molecular expression of myostatin and MyoD is greater in double-muscled than normal-muscled cattle fetuses. Am J Physiol Regulatory Integrative Comp Physiol. 280: R1488-R1493.
Sodiq A, Suwarno, Fauziyah FR, Wakhidati YN, Yuwono P. 2017. Sistem produksi peternakan sapi potong di pedesaan dan strategi pengembangannya. Agripet. 17(1):60-66.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis versions 6.0. Mol Biol Evol. 30(12):2725-2729.
Yulianto P, Saparinto C. 2014. Beternak Sapi Limousin; Panduan pembibitan, pembesaran dan penggemukan. Penebar Swadaya. Semarang.
Zhang C, Liu Y, Xu D, Wen Q, Li X, Zhang W, Yang L. 2011. Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on boer goat growth performance. Mol Biol Rep. 39(3):3081-3087.
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Copyright (c) 2022 Wenny Ladhunka Nur Aliyya, Jakaria Jakaria, Ronny Rachman Noor
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