Aktivitas Antibakteri Ekstrak Akar Ceplukan (Physalis angulata L.) Terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa
DOI:
https://doi.org/10.37090/jfl.v6i2.20Abstrak
Root ceplukan (Physalis angulata L.) is one of the medicinal plants that contain several active compounds that are antibacterial. This study aims to prove the antibacterial activity of root ceplukan extract againsts Staphylococcus epidermidis bacteria and Pseudomonas aeruginosa bacteria. Root ceplukan was extracted by maceration with ethanol 70%. Root root extracts of phytochemical screening include flavonoids, alkaloids and saponins. The ceplukan root extract was then tested against S. epidermidis and P. aeruginosa bacteria using wells method with concentrations of 20%, 40%, 60%, 80%, 100%, aquades as a negative control and amikacin as a positive control. Inhibitory zone diameter with each concentration for P. aeruginosa bacteria, among others, negative control = 0 mm, positive control = 21,623 mm, 100% = 16.9 mm, 80% = 15.05 mm, 60% = 13.703 mm, 40% = 12.02 mm, 20% = 12.02 mm and S. epidermidis bacteria have inhibitory zone at positive control of 21,623 mm. The results of this study showed that root extract ceplukan proved to have inhibition zone against bacteria P. aeruginosa at all concentrations but has no inhibition zone against S. epidermidis bacteria. Root ceplukan extract has a minimum inhibitory concentration of 14% and a minimum bacterisid concentration of 20%, against P. aeruginosa bacteria. The results of phytochemical analysis showed that root ceplukan positively contain flavonoids, alkaloids and saponins are suspected as antibacterial compounds. Keywords : Antibacterial, Staphylococcus epidermidis, Pseudomonas aeruginosa, Ceplukan root extractReferensi
Jawetz E, Melnick J.L, Adelberg A. 1996. Mikrobiologi Kedokteran. 21st ed. Jakarta: Buku Kedokteran EGC.
Permatasari C.H. 2010. Pengaruh Daya Antibakteri Obat Kumur Ekstrak Etanol Daun Ciplukan ( Physalis angulata L .) Terhadap Bakteri Staphylococcus aureus In Vitro. Program Studi Pendidikan Dokter Gigi Fakultas Kedokteran dan Ilmu Kesehatan Universita. Dapartemen Biomedis Program Studi Pendidik Dr Gigi.
Karyono S.S. 2011. Efektivitas Ekstrak Daun Ceplukan sebagai Antimikroba terhadap Methicillin-Resistant Staphylococcus aureus In Vitro Effectiveness of Ceplukan Leaf Extract as Antimicrobial for Methicillin-Resistant Staphylococcus aureus In Vitro. 26(4):212–215.
Vitasari ON. 2012. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Ceplukan (Physalis angulata L.) Terhadap Staphylococcus aureus Dan Pseudomonas aeruginosa. Universitas Sebelas Maret Surakarta.
Hidayah V.F.N. 2009. Pengaruh Pemberian Akar Ceplukan (Physalis Angulata L.) Terhadap Kerusakan Hepatosit Akibat Pemberian Parasetamol Pada Mencit (Mus Musculus. Universitas Muhammadiyah Surakarta.
Shingu K. 1992. There New Withanolides, Physagulins E, F and G from Physalis angulata L. 2448– 2451.
Marliana, S. D., Suryanti V., Suyono. 2005. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimai Labu Siam (Sechium edule Jacq. Swartz) dalam Ekstrak Etanol. Biofarmasi 3(1) : 26 – 31.
Sangi M., Runtuwene M.R.J., Simbala H.E.I., Makang V.M.A. 2008. Analisis Fitokimia Tumbuhan Obat di Kabupaten Minahasa Utara. Chem. Prog. 1 (1): 47 – 53.
Pelczar C. 1986. Dasar-dasar mikrobiologi II. Diterjemahkan, RS oleh H, T I, Tjit, SS, Rosomo, et al., editors. Jakarta: Universitas Indonesia. 489-522.
Taufik A.S. 2014. Uji Efektivitas Ekstrak Daun Salam (Eugenia polyantha) Terhadap Pertumbuhan Staphylococcus aureus Secara In vitro. Skripsi. Universitas Hasanuddin Makassar.
Nuria M.C., Faizatun A, Sumantri. 2009. Uji Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha cuircas L) terhadap bakteri Stphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408. Ilmu Pertanian 26–37.
